Fernando took part in light training ahead of the match but his groin problem will see him absent from the squad, as is fellow midfielder Samir Nasri who is recovering from groin surgery.
Manager Manuel Pellegrini has indicated Joe Hart will return in goal in place of Willy Caballero.
Hart has admitted he finds it "hard to see the positives" of being rested and said he is relishing the challenge of fighting for his place.
The England goalkeeper was replaced by summer signing Caballero for the 4-2 win at Hull at the weekend, but is set to be recalled.
Hart said last week he was in the dark over a new contract with City - his current deal has just under two years left to run - but insists he wants to stay at the club for as long as he is welcome.
The 27-year-old was dropped last season after run of errors, but reclaimed his place, and conceded he found it difficult to see any positives from being rested.
Hart told a news conference: "It is hard for me to see it that way
I am sure when I retire from football I will see them, but right now I am not going to see them.
"Of course I want to play every game, that is my nature, my professionalism
I am not the manager, just a player
I live by the decisions of the manager.
"We have always had good goalkeepers, David James, [Andreas] Isaksson, Kasper [Schmeichel], [Costel] Pantilimon, it has always been a fight for me to keep my place and that will continue, but I have always had high standards that I will keep to, whether there is one goalkeeper or 20
I love the challenge."
The match at the Etihad will see Hart come up against his former England team-mate Ashley Cole, who moved from Chelsea to Italy in the summer
Micah Richards, another City and England team-mate has also moved to Serie A with Fiorentina, but Hart said he had no urge to leave.
"I have got a lot of respect for the boys who have done, Micah being closest to home for me," said Hart
"He seems to be loving it out there, but right now I am happy with what is going on at this club
We are fighting on all fronts
While I am welcome here, I am more than happy to stay here and see where that takes me."
Neither Hart nor Manuel Pellegrini would be drawn on his contract situation, however, with the City manager stating: "Me and Joe know what happen with that issue
It is important but not the most important thing in this moment."
City's opponents Roma have begun the season in breathtaking form, winning all five of their matches in Serie A and thrashing Champions League group rivals CSKA Moscow 5-1
That heaps the pressure on City to get a win, especially after Pellegrini's side conceded a last-minute winner to Bayern Munich in their opening European match.
Hart added: "It is obviously a big game after a disappointing start in Munich
We are aware it is the Champions League and not the knock-out phase straight away
We have to build on it especially at home and we are going in trying to win the game.
"They [Roma] have been doing very well
We have focused a lot more on them since drawn in our group, they got a great result in matchday one
Every game is tough in Champions League, but we are up for it and more than capable of winning."
Late goals have proved costly for City in Europe, with Bayern, Barcelona last season and Real Madrid in 2012 all scoring late on to secure victories
Hart, however, says City can cope with the "big boys" of European football.
He said: "Of course, those have cost us the games in some big games, but also shown that we are definitely up there and can play with the big boys and we just need to finish the game off.
"It is pretty much standard for the Champions League
We have always had tough groups in our brief Champions League history
They are big nights
We all understand you have to do well at home."
Pellegrini pointed out that City lost their opening group match last season, also against Bayern, but went on to win all the other five and qualify from the group.
He said: "It is normal pressure
Remember last year we start here against Bayern at home and we lost at home
Maybe that is more pressure
But we lost the first game and won the other five games."
Roma owner James Pallotta claims the game will be "a blast" - and that the Premier League champions had been anxious to avoid the Italian club in the draw.
Pallotta, a hugely-wealthy Italian-American who made is fortune in hedge funds, insisted Roma can mirror Atletico Madrid's run last season as an unfancied team making it all the way to the final
He told Press Association Sport: "I think it's going to be a blast
We had a conversation with Manchester City and they didn't want us in the draw either!
"If you can't go through teams like that to get to the next round then I don't think you deserve to be in the Champions League anyway.
"I think we have a great team and it's going to be a lot of fun.
"Our goal is to go all the way
Look at Atletico Madrid last year they were about four minutes away from winning it and no one would have even thought they had a chance at the beginning of the year, not even winning their league.
"Anything can happen, it depends on how hungry we are."
Source : PA