The Eastlands club have also expressed their disappointment at the "actions of his representative" over the matter. The relationship between Tevez and City manager Roberto Mancini has reportedly deteriorated in recent weeks although the player is understood to be unhappy at being apart from his two daughters who live in Argentina.
A statement from the club read: "It is with disappointment that we confirm to our supporters that Carlos Tevez has submitted a written transfer request. The club can also confirm that the request has been rejected.
"The club remains disappointed by this situation and particularly with the actions of Carlos' representative.
"Roberto Mancini and all at the club have shown, and will continue to show, sensitivity to Carlos' personal circumstances including the issue of his family being based overseas.
"Indeed following his suspension as a result of the game against Bolton, Carlos requested, and was given, special dispensation by the manager to take leave overseas. The written transfer request is in stark contrast to Carlos' stated position in both public and club contexts."